TABLE 5

Sulfoxidation activity toward sulprofos with P450 and FMO isoforms Values are the mean ± S.E.M. (n = 3).




Km

Vmax

CLint

%TNR
μM nmol/nmol μl/nmol
isoform/min isoform/min
1A1 17.0 ± 1.4 7.40 ± 0.2 430 N.D.
1A2 11.0 ± 0.9 6.47 ± 0.1 590 14.6
3A4 27.0 ± 3.0 13.0 ± 0.5 480 28.6
3A5 12.9 ± 2.0 5.22 ± 0.2 400 0.2
2C9*1 13.6 ± 2.9 11.4 ± 0.7 840 44.4
2C9*2 10.2 ± 1.8 7.83 ± 0.3 770 N.D.
2C9*3 6.8 ± 1.0 5.15 ± 0.2 760 N.D.
2C18 7.00 ± 1.0 4.40 ± 0.2 630 0.9
2C19 14.0 ± 3.0 10.6 ± 0.7 780 8.2
2D6*1 24.8 ± 1.7 11.1 ± 0.2 450 2.5
FMO1
14.4 ± 2.5
16.1 ± 0.8
1120
0.6
  • N.D., not determined.