TABLE 4

CLint, Vmax, and KMvalues for substrates and metabolites in fresh and cryopreserved suspensions (data are the mean ± SD of three determinations)


Metabolite

CLint

Vmax

KM or S50§
Fresh Suspension
Cryopreserved Suspension
Fresh Suspension
Cryopreserved Suspension
Fresh Suspension
Cryopreserved Suspension
μl/min/106 cells nmol/min/106 cells μM
HTOL 0.29 ± 0.05 0.47 ± 0.19 0.39 ± 0.08 0.13 ± 0.04* 1390 ± 490 300 ± 140*
7-HCa 38.6 ± 6.5 9.8 ± 1.0* 0.0064 ± 0.0013 0.0034 ± 0.0005* 0.18 ± 0.06 0.37 ± 0.07*
4-OH PHEa 12.0 ± 2.3 5.4 ± 1.4* 0.087 ± 0.036 0.024 ± 0.002* 7.6 ± 1.9 4.7 ± 1.7
4′-OH NDZ 14 ± 5b 0.53 ± 0.30* 0.228 ± 0.050b 0.0067 ± 0.0039* 18 ± 7b 24.7 ± 1.3
OXP 2.45 ± 0.69b 0.33 ± 0.14* 0.875 ± 0.126b 0.182 ± 0.137* 209 ± 45§b 287 ± 177§
NDZ Total 16.2 ± 5.1b 0.86 ± 0.42* - - - -
4′-OH DZ 52.8 ± 18.3 31.9 ± 15.4 0.20 ± 0.04 0.13 ± 0.05 4.4 ± 2.2 4.1 ± 0.9
NDZ 11.9 ± 2.3 7.2 ± 1.6 0.40 ± 0.10 0.59 ± 0.06* 36 ± 14 86 ± 26*
TZ 25.5 ± 10.7 16.2 ± 3.1 1.45 ± 0.53 2.94 ± 1.50 59 ± 22 200 ± 146
DZ Total 90.1 ± 22.5 55.3 ± 17.3 - - - -
DOR 54.3 ± 15.1 8.5 ± 2.3* 0.69 ± 0.06 0.41 ± 0.17* 9.2 ± 1.6§ 48 ± 18*
MEM 43.2 ± 8.5 3.3 ± 2.4* 1.34 ± 0.20 2.63 ± 2.35 21 ± 6§ 454 ± 280§*
DEX Total 97.6 ± 19.0 11.8 ± 4.0* - - - -
4-OH WAR 0.011 ± 0.0027 0.045 ± 0.030 0.0063 ± 0.0032 0.0059 ± 0.0018 542 ± 167 210 ± 186
6-OH WAR 0.0068 ± 0.0028 0.028 ± 0.010* 0.0018 ± 0.0010 0.0028 ± 0.0004 261 ± 68 113 ± 57*
7-OH WAR 0.063 ± 0.021 0.03 ± 0.013 0.0053 ± 0.0023 0.0070 ± 0.0037 83 ± 15 293 ± 198
8-OH WAR 0.012 ± 0.0015 0.127 ± 0.008* 0.018 ± 0.006 0.043 ± 0.012* 1452 ± 424 339 ± 112*
10-OH WAR 0.004 ± 0.0016 0 0.0008 ± 0.0002 0 240 ± 159 0
WAR Total 0.097 ± 0.028 0.229 ± 0.040* - - - -
PROP
49 ± 8
39 ± 15
ND
ND
ND
ND
  • a CLint values are for both sites, Vmax and KM values are for the high-affinity site only

  • b Incubations for NDZ in fresh suspensions performed by A. Parker (personal communication)

  • * Significant difference between fresh and cryopreserved suspensions (P < 0.05)

  • § S50 calculated