Incubation conditions for substrates in fresh and cryopreserved rat hepatocyte suspensions
Substrate | Metabolite | Substrate concentration in incubation | Fresh Suspension | Cryopreserved Suspension | ||||
|---|---|---|---|---|---|---|---|---|
| Cell density | Incubation time | Cell density | Incubation time | |||||
| μM | 106 cells/ml | min | 106 cells/ml | min | ||||
| TOL | HTOL | 100-1500 | 1 | 15 | 0.5 | 90 | ||
| 7-EC | 7-HC | 0.05-200 | 0.5 | 15 | 0.5 | 60 | ||
| PHE | 4-OH PHE | 0.1-100 | 1 | 15 | 0.375 | 60 | ||
| NDZ | 4′-OH NDZ | 0.5-500 | NDa | NDa | 0.375 | 60 | ||
| OXP | ||||||||
| DZ | 4′-OH DZ | 2.5-250 | 0.5 | 10 | 0.375 | 30 | ||
| NDZ | ||||||||
| TZ | ||||||||
| DEX | DOR | 1-500 | 0.5 | 10 | 0.5 | 60 | ||
| MEM | ||||||||
| WAR | 4-OH WAR | 25-1500 | 2 | 60 | 1 | 60 | ||
| 6-OH WAR | ||||||||
| 7-OH WAR | ||||||||
| 8-OH WAR | ||||||||
| 10-OH WAR | ||||||||
| PROP | -b | 5 | 0.2,1 | Up to 60 | 025, 0.375, 0.5 | Up to 120 | ||