TABLE 1

Fluorimetric probes for P450 activity assays


Substrate

Metabolite

Ex/Em

Quenching Solution

Reference
nm
CEC Cyano-hydroxycoumarin 408/455 0.1 M phosphate potassium buffer, pH 7.4 White (1988)
Coumarin 7-Hydroxycoumarin 355/460 0.1 M Tris, pH 9 Donato et al. (1998)
BFC HFC 410/510 0.25 M Tris in 60% (v/v) acetonitrile Price et al. (2000)
EFC HFC 410/510 0.25 M Tris in 60% (v/v) acetonitrile Buters et al. (1993)
MFC HFC 410/510 0.25 M Tris in 60% (v/v) acetonitrile Bapiro et al. (2001)
DBF Fluorescein 485/538 2 N sodium hydroxide Stresser et al. (2000)
AMMC
AHMC
390/460
0.25 M Tris in 60% (v/v) acetonitrile
Chauret et al. (2001)
  • Ex/Em, excitation and emission wavelengths; AHMC, 3-[2-(diethylamino)ethyl]-7-hydroxy-4-methylcoumarin.