Experimental conditions and kinetic characteristics of fluorescent probe assays in intact P450-expressing cells
P450 | Substrate | Incubation Time | Concentration Assay | Km | Vmax b |
|---|---|---|---|---|---|
| min | μM | μM | pmol/min/mg | ||
| 1A2 | CEC | 90 | 30 | 5.8 ± 2.8 | 64.3 ± 5.0 |
| 2A6 | Coumarin | 45 | 50 | 9.1 ± 1.3 | 349 ± 75 |
| 2B6 | EFC | 60 | 30 | 4.2 ± 2.8 | 35.1 ± 4.9 |
| 2C8 | DBF | 120 | 10 | 1.9 ± 0.6 | 1.3 ± 0.5 |
| 2C9 | MFC | 120 | 150 | 50.7 ± 4.1 | 11.5 ± 2.7 |
| 2C19 | CEC | 90 | 60 | 20.3 ± 4.7 | 1.7 ± 0.3 |
| 2D6 | AMMC | 120 | 100 | 19.2 ± 1.5 | 0.95 ± 0.2 |
| 2E1 | MFC | 60 | 10 | 2.1 ± 1.0 | 12.1 ± 3.0 |
| 3A4 | BFC | 60 | 100 | 25.6 ± 9.2 | 2.4 ± 0.9 |
aOptimal substrate concentration for obtaining maximal activity.
↵ b pmol of metabolite formed per minute and per mg of total cell protein