TABLE 3
Kinetic parameters of 5-FU formation from capecitabine in the combination of human liver microsomes and cytosol, microsomes, and cytosol Capecitabine (0.05—3 mM) was incubated with the combination of microsomes and cytosol (1.2 mg/ml), microsomes (0.2 mg/ml), and cytosol (1.0 mg/ml) obtained from BD Gentest for 30 min in the presence of CDHP. The kinetic parameters were calculated from the fitted curves by nonlinear regression. The detection limit of 5-FU was 1.0 pmol.