TABLE 4

Cytochrome P450 Activity (nmol/min) with respect to diazepam 4'-hydroxylation and 7-ethoxycoumarin O-deethylase activity in rat hepatocytes

Values are mean ± S.D. (n = 4).

Time (min)
Diazepam
7-Ethoxycoumarin
0.5 × 106 cells/ml
1 × 106 cells/ml
0.5 × 106 cells/ml
1 × 106 cells/ml
0 0.067 ± 0.021 (100)a 0.13 ± 0.039 (100) 0.050 ± 0.0044 (100) 0.094 ± 0.0071 (100)
30 0.068 ± 0.022 (101) 0.12 ± 0.035 (93) 0.054 ± 0.0072 (107) 0.11 ± 0.011 (114)
60 0.064 ± 0.022 (95) 0.11 ± 0.033 (85) 0.054 ± 0.0062 (108) 0.11 ± 0.0095 (112)
120 0.051 ± 0.013 (76) 0.088 ± 0.026 (80) 0.051 ± 0.0084 (102) 0.094 ± 0.011 (100)
240
0.038 ± 0.010b (56)
0.085 ± 0.028b (65)
0.042 ± 0.0053 (85)
0.076 ± 0.0087b (65)

  • ↵ a Numbers in parentheses represent a percentage of the time = 0 value.

  • ↵ b Significantly different from the corresponding time = 0 value (p < 0.05, Dunnett's test).