Kinetic analysis of etoposide catechol formation by recombinant human P450s
The kinetic constants were determined according to Lineweaver-Burk double-reciprocal plots. The values (Km and Vmax) are reported as mean ± S.D. (n = 3).
P450s | Km | Vmax | Vmax/Km |
---|---|---|---|
μM | pmol/min/nmol P450 | μl/nmol P450/min | |
CYP3A4 | 77.7 ± 27.8a | 314 ± 84b | 4.0 |
CYP3A5 | 13.9 ± 3.1c | 19.4 ± 0.4d | 1.4 |
CYP2E1 | 26.0 ± 10.5 | 3.4 ± 1.2 | 0.1 |