TABLE 1

Primers and probes sequences for real-time quantitative PCR (TaqMan)


CYP3A4 (GenBank accession number AF182273)

Forward primer (631-657): 5′-AAGAAGCTTTTAAGATTTGATTTTTTG-3′
Reverse primer (730-703): 5′-ACACAGATATTTAATACTTCAAGAAT-3′
Probe (677-698): 5′-TAACAGTCTTTCCATTCCTCAT-3′ (MGB)
CYP3A5 (GenBank accession number NM_000777) Forward primer (258-279): 5′-CTGGAAATTTGACACAGAGTGC-3′
Reverse primer (403-377): 5′-TTCGATTTGTGAAGACAGAATAACATT-3′
Probe (306-334): 5′-AACGTATGAAGGTCAACTCCCTGTGCTGG-3′
CYP3A7 (GenBank accession number NM_000765) Forward primer (636-661): 5′-AAGATTTAATCCATTAGATCCATTCG-3′
Reverse primer (782-754): 5′-CGACCTTCTTTTATCTGTTTTACAGATTT-3′
Probe (674-707): 5′-AAGTCTTTCCATTCCTTACCCCAATTCTTGAAGC-3′
P-glycoprotein (P-gp) (GenBank accession number M14758) Forward primer (3103-3123): 5′-TGGGAAGATCGCTACTGAAGC-3′
Reverse primer (3226-3201): 5′-TTTCCTCAAAGAGTTTCTGTATGGTA-3′
Probe (3131-3161): 5′-AACTTCCGAACCGTTGTTTCTTTGACTCAGG-3′
Villin (GenBank accession number XM_010866) Forward primer (1042-1061): 5′-GCCGTCTTTCAGCAGCTCTT-3′
Reverse primer (1143-1124): 5′-CACCTGTTCCACTTTGGCCA-3′

Probe (1063-1086): 5′-CAGAAGTGGACAGCGTCCAACCGG-3′