Experimental conditions for P450 activity assays
P450 | Reaction | Conc. Substrate | Incubation Time | Detection Technique |
|---|---|---|---|---|
| μM | min | |||
| 1A2 | 7-Methoxyresorufin O-demethylation (MROD) | 10 | 60 | Fluorimetry (Donato et al., 1993) |
| 2A6 | Coumarin 7-hydroxylation (COH) | 50 | 60 | Fluorimetry (Donato et al., 1998) |
| 2C9 | Diclofenac 4′-hydroxylation (D4OH) | 300 | 180 | HPLC analysis (Bort et al., 1999) |
| 2C19 | Mephenytoin 4′-hydroxylation (M4OH) | 200 | 180 | HPLC analysis (Bort et al., 1999) |
| 2E1 | Chlorzoxazone 6-hydroxylation (C6OH) | 400 | 180 | HPLC analysis (Bort et al., 1999) |
| 3A4 | Testosterone 6β-hydroxylation (6β-OHT) | 200 | 60 | HPLC analysis (Donato et al., 1995) |
| Various | 7-Ethoxycoumarin O-deethylation (ECOD) | 800 | 60 | Fluorimetry (Donato et al., 1999) |