TABLE 3

Individual P450 activities in human hepatocytes prepared from CS-preserved liver grafts

Data are expressed as picomole(s) of product formed per minute and per milligram of total cell protein.

MROD
COH
D4OH
M4OH
C6OH
6β-OHT
Culture CYP1A2 CYP2A6 CYP2C9 CYP2C19 CYP2E1 CYP3A4
1 N.D. N.D. 25.7 1.1 19 26.3
2 0.02 9.1 55.0 4.5 110 4.6
3 0.58 N.D. 21.6 23.5 113 20.9
4 0.36 14.9 11.0 4.5 55 48.0
5 1.85 4.8 59.5 10.8 244 80.5
6 0.48 19.1 10.8 3.8 86 23.9
Mean 0.55 12.0* 30.6* 8.0 105 34.0
S.D. 0.68 6.3 21.5 8.2 77 26.7
Variationa >90 3 5 20 12 17
SLR
Mean (n = 7) 1.04 36.2 84.9 29.2 175 53.8
S.D. 0.63 20.5 40.7 27.2 111 40.1
Variation
8
4
3
10
7
10

  • COH, coumarin 7-hydroxylation; D4OH, diclofenac 4′-hydroxylation; M4OH, mephenytoin 4′-hydroxylation; C6OH, chlorzoxazone 6-hydroxylation; N.D., not determined.

  • ↵* P < 0.05 with respect to SLR group.

  • ↵ a Variation = (highest value - lowest value)/lowest value.