TABLE 4

Dilutions of monoclonal antibodies that exhibited maximum specificity for inhibition of human liver microsomal metabolism of P450 marker substrates


P450 Isoform

Marker Substrate

Reaction Catalyzed

Maximum % Inhibition

Dilution of Stock Solution
1A2 Phenacetin Phenacetin-O-deethylation 80 1:800
2C9 Tolbutamide Tolbutamide hydroxylation 65 1:240
2C19 S-Mephenytoin S-Mephenytoin 4′-hydroxylation 96 1:240
2D6 Dextromethorphan Dextromethorphan O-demethylation 90 1:40
3A4
Testosterone
Testosterone 6β-hydroxylation
87
1:20