TABLE 1

Apparent kinetic parameters for the formation of 2-hydroxyiminostilbene in human liver microsomes and insect cell microsomes containing recombinant CYP3A4

Values are presented ± standard error.


Sample

2-Hydroxyiminostilbene Formation
Km
Vmax
Clint
μM
H56 218 ± 66 42 ± 6a 0.19b
H30 204 ± 17 90 ± 4a 0.44b
H42 141 ± 38 294 ± 39a 2.09b
Pool 104 ± 16 65 ± 4a 0.62b
CYP3A4
124 ± 10
2.0 ± 0.1c
16d
  • a pmol/mg protein/min.

  • b μl/mg protein/min.

  • c pmol/pmol P450/min.

  • d μl/nmol P450/min.