Inhibitory effects of anions and cations on the uptake of [3H]bumetanide by MCT6
Uptake rate of 0.1 μM [3H]bumetanide inMCT6-expressing oocytes or uninjected oocytes was measured at room temperature and pH 7.4 or pH 6.0 for 30 min in the presence or absence of inhibitor. MCT6-specific uptake was obtained by subtracting the uptake amount of uninjected oocytes from that of MCT6-expressing oocytes. The results are shown as percentages of control uptake measured in the absence of inhibitor. Each value represents the mean ± S.E.M. (n = 4–10).
Inhibitor | Concentration | MCT6-Specific Uptake | ||
|---|---|---|---|---|
| pH 7.4 | pH 6.0 | |||
| mM | % of control | |||
| l-Lactic acid | 5 | 123.8 ± 11.4 | 76.8 ± 11.7 | |
| Pyruvic acid | 5 | 95.1 ± 12.4 | 75.7 ± 5.6 | |
| Succinic acid | 5 | 101.8 ± 14.2 | 73.0 ± 7.3 | |
| Citric acid | 5 | 128.2 ± 8.9 | 99.2 ± 0.5 | |
| Choline | 5 | 126.4 ± 14.1 | 76.3 ± 6.2 | |
| Cimetidine | 5 | 40.4 ± 2.6* | 58.1 ± 7.9* | |
| p-Aminohippuric acid | 5 | 30.9 ± 7.7* | 83.9 ± 1.9 | |
| Estrone-3-sulfate | 5 | 11.6 ± 4.0* | 21.3 ± 8.1* | |
| Chlorothiazide | 0.5 | 52.0 ± 6.7* | 77.5 ± 9.7 | |
| Hydrochlorothiazide | 0.5 | 71.0 ± 5.1* | 68.6 ± 5.1* | |
| Trichlormethiazide | 0.5 | 20.7 ± 2.6* | 64.2 ± 5.5* | |
| Probenecid | 0.5 | 46.3 ± 2.4* | 82.6 ± 14.2 | |
| Glibenclamide | 0.1 | 31.3 ± 4.3* | 56.3 ± 9.4* | |
| Nateglinide | 0.001 | 40.8 ± 2.5* | 47.1 ± 10.3* | |
↵* p < 0.05 vs. control.