Primers and probes
Primers for cDNA Standards | ||||
|---|---|---|---|---|
| Gene | Forward Primer | Reverse Primer | ||
| MDR1 (ABCB1) | 5′-ACAGTCCAGCTGATGCAGAGG-3′ | 5′-CCTTATCCAGAGCCACCTGAAC-3′ | ||
| MRP1 (ABCC1) | 5′-CACACTGAATGGCATCACCTTC-3′ | 5′-CCTTCTCGCCAATCTCTGTCC-3′ | ||
| MRP2 (ABCC2) | 5′-CCAATCTACTCTCACTTCAGCGAGA-3′ | 5′-AGATCCAGCTCAGGTCGGTACC-3′ | ||
| MRP3 (ABCC3) | 5′-TCTATGCAGCCACATCACGG-3′ | 5′-GTCACCTGCAAGGAGTAGGACAC-3′ | ||
| MRP4 (ABCC4) | 5′-AAGTGAACAACCTCCAGTTCCA-3′ | 5′-CCGGAGCTTTCAGAATTGAC-3′ | ||
| MRP5 (ABCC5) | 5′-CTAGAGAGACTGTGGCAAGAAGAGC-3′ | 5′-AAATGCCATGGTTAGGATGGC-3′ | ||
| Villin (VIL1) | 5′-AGAAAGCCAATGAGCAGGAGAA-3′ | 5′-ATGGATGTGGCATCGAACTTC-3′ | ||
| Primers and Probes for TaqMan Analysis | |
|---|---|
| Gene | Probe |
| MDR1 (ABCB1) | 5′-AAGCTGTCAAGGAAGCCAATGCCTATGACTT-3′ |
| MRP1 (ABCC1) | 5′-CCTCCACTTTGTCCATCTCAGCCAAGAG-3′ |
| MRP2 (ABCC2) | 5′-CTCAATATCACACAAACCCTGAACTGGCTG-3′ |
| MRP3 (ABCC3) | 5′-CCAACCGGTGGCTGAGCATCG-3′ |
| MRP4 (ABCC4) | 5′-CAAACCGAAGACTCTGAGAAGGTACGATTCCT-3′ |
| MRP5 (ABCC5) | 5′-CTGACGGAAATCGTGCGGTCTTGG-3′ |
| Villin (VIL1) | 5′-TCATCAAAGCCAAGCAGTACCCACCAAG-3′ |
| Gene | Forward Primer |
| MDR1 (ABCB1) | 5′-CTGTATTGTTTGCCACCACGA-3′ |
| MDR1 (ABCB1) | 5′-CTGTATTGTTTGCCACCACGA-3′ |
| MRP1 (ABCC1) | 5′-GGGCTGCGGAAAGTCGT-3′ |
| MRP2 (ABCC2) | 5′-ACTGTTGGCTTTGTTCTGTCCA-3′ |
| MRP3 (ABCC3) | 5′-GGTGGATGCCAACCAGAGAA-3′ |
| MRP4 (ABCC4) | 5′-AAGTGAACAACCTCCAGTTCCAG-3′ |
| MRP5 (ABCC5) | 5′-CTGCAGTACAGCTTGTTGTTAGTGC-3′ |
| Villin (VIL1) | 5′-CATGAGCCATGCGCTGAAC-3′ |
| Gene | Reverse Primer |
| MDR1 (ABCB1) | 5′-AGGGTGTCAAATTTATGAGGCAGT-3′ |
| MRP1 (ABCC1) | 5′-AGCCCTTGATAGCCACGTG-3′ |
| MRP2 (ABCC2) | 5′-CAACAGCCACAATGTTGGTCTCTA-3′ |
| MRP3 (ABCC3) | 5′-GCAGTTCCCCACGAACTCC-3′ |
| MRP4 (ABCC4) | 5′-GGCTCTCCAGAGCACCATCT-3′ |
| MRP5 (ABCC5) | 5′-TCGGTAATTCAATGCCCAAGTC-3′ |
| Villin (VIL1) | 5′-TCATTCTGCACCTCCACCTGT-3′ |