TABLE 1

Kinetic parameters for the transport of LTC4 and E217ßG by rat and dog Mrp2

Each value was calculated from the data shown in Fig. 6. Values represent the obtained parameter ± computer calculated S.D. Vmax and PS′ns values mean Vmax and PSns values normalized by the absolute expression amount of each Mrp2 protein, respectively.




LTC4

E217βG
Rat Dog Rat Dog
Vmax (nmol/min/mg protein) 1.55 ± 0.05 1.18 ± 0.01 0.39 ± 0.02 0.06 ± 0.01
Km (μM) 2.05 ± 0.41 0.53 ± 0.03 4.81 ± 1.21 3.25 ± 0.10
PSns (μl/min/mg protein) 22.7 ± 0.41 34.7 ± 0.23
n 1.16 ± 0.22 1.30 ± 0.04
Vmax (nmol/min/nmol Mrp2) 2.97 ± 0.10 2.88 ± 0.02 0.75 ± 0.04 0.14 ± 0.02
PS′ns (μl/min/nmol Mrp2) 43.3 ± 0.78 84.9 ± 0.55
Vmax/Km (μl/min/nmol Mrp2)
1.45 × 103
5.48 × 103
1.56 × 102
0.45 × 102