Kinetic constants of CBR3 V244 and CBR3 M244
CBR3 V244 | CBR3 M244 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Km | Vmax | kcat | Vmax/Km | Km | Vmax | kcat | Vmax/Km | |||||||
| μM | μmol/min · mg | s-1 | min-1 × 103 | μM | μmol/min · mg | s-1 | min-1 × 103 | |||||||
| Menadionea | 24.6 ± 3.2 | 19.6 ± 2.0 | 9.9 | 797 | 22.9 ± 2.9 | 40.6 ± 1.3 | 20.5 | 1773 | ||||||
| NADP(H)b | 90.5 ± 2.5 | 32.4 ± 1.0 | 16.3 | 358 | 85.0 ± 2.0 | 53.0 ± 1.0 | 26.7 | 624 | ||||||
↵ a Each value corresponds to the mean ± S.D. obtained from eight experiments performed with two independent protein preparations for each isoform
↵ b Menadione was held constant at 150 μM. Each value represents the mean ± S.D. obtained from four experiments with two independent protein preparations