TABLE 1

Kinetic parameters for paclitaxel 6α-hydroxylase activities of wild-type and variant CYP2C8

Each value represents the mean ± S.E.M. of three independent transfection experiments.


CYP2C8

Km

Vmax

Vmax/Km
pmol/min/mg protein
pmol/min/pmol P450
μl/min/mg protein
μl/min/pmol P450
μM
Wild-type 7.08 ± 0.30 87.4 ± 8.6 32.4 ± 3.4 12.5 ± 1.7 4.60 ± 0.54
G171S 7.80 ± 0.13 96.4 ± 13.7 37.7 ± 4.9 12.4 ± 1.9 4.85 ± 0.70
K247R 6.45 ± 0.83 76.1 ± 8.5 32.7 ± 6.1 11.9 ± 1.2 5.04 ± 0.51
K383N
6.44 ± 0.26
57.6 ± 5.6
36.1 ± 2.9
8.97 ± 0.95
5.66 ± 0.63