Kinetic parameters of wild-type and L48V UGT1A4 for trans-androsterone, imipramine, cyproheptadine, and tigogenin
Substrate | Parameter | Wild Type | L48V |
|---|---|---|---|
| trans-Androsterone | Vmaxa (pmol/min/mg) | 107.3 | 35.7 |
| Kmb (μM) | 254.7 | 8.2 | |
| Imipramine | Vmax (pmol/min/mg) | 872.8 | 294.9 |
| Km (μM) | 1442.0 | 275.8 | |
| Cyproheptadine | Vmax (pmol/min/mg) | 342.6 | 203.3 |
| Km (μM) | 227.5 | 86.4 | |
| Tigogenin | Vmax (pmol/min/mg) | 158.6 | 81.4 |
|
| Km (μM) | 7.8 | 6.7 |