TABLE 1

PCR primers for house-keeping gene and human UGT1A family Some primers were designed based on the work of Tukey and his coworkers (e.g., Strassburg et al, 1999).

Fragment	Accession No. (GenBank)	Sequence (5′-3′)	Position	Expected Size
			bases
β-Actin	M10278	GGCGGCACCACCATGTACCCT	942–962
		CGATCCACACGGAGTACTTGC	1045–1065	124
UGT1	M57899	TCGAATCTTGCGAACAACACG	1042–1062
		ATCATCACCATGGGAACGCC	1168–1187	146
1A1	M57899	AACAAGGAGCTCATGGCCTCC	412–432
		CATGCAAGAAGAATACAGTGG	515–535	124
1A3	M84127	TGGTCTATCATAGGTCTTGTG	495–515
		AACCACATCAAAGGAAGTAGC	557–577	83
1A4	M57951	GAAGGAATTTGATCGCGTTAC	287–307
		GGCCTCATTATGCAGTAGCTC	417–437	151
1A5	M84129	CCCTGGAGGTGAATATGTA	299–317
		AGAACGATTGAGTGTGACC	397–415	117
1A6	M84130	GGCCTACCATCTGTGTACCTC	703–723
		TAGGACACAGGGTCTGGGCT	766–785	83
1A7	U39570	GGACGGCACCATTGCGAAG	458–476
		CAAACTCCTGCAATTTAA	538–556	99
1A8	U42604	GGAATAGGTTGCCACTATCT	774–793
		AGTCATGGCATCTGAGAACC	850–869	96
1A9	S55985	GGAACATTTATTATGCCACCG	679–699
		TGGCTGTAGAGATCATACTCC	754–774	96
1A10	U39550	CCAATGATCTCTTAGGGTTCT	851–871
		AATGGTCCTCCAAGTGCACGA	978–998	148
1A3-5	M57951	ACATGCTCTACCCTCTGGCCC	667–687
		CACCACTGACACCTCTCTCTG	747–767	101
1A7-10	S55985	CAGTGCCCTGCTCCTCTTTCC	578–598
		AAGTGCATGATGTGGTTCCGT	655–675	98