TABLE 2

Apparent kinetic parameters for the glucuronidation of fluoroquinolones by human liver microsomes For Km and Vmax values, glucuronidation activities from triplicate incubations were averaged and nonlinear regression was used to fit the data to the Michaelis-Menten equation. The parameter estimated and the S.E. value generated by the GraFit 5.0 software are shown.


Compound

Km

Vmax

Vmax/Km
mM pmol/min/mg protein (μl/min/mg) × 100
LVFX 10.0 ± 1.2 27.8 ± 3.12 0.28
GPFX 5.2 ± 0.87 115 ± 13.0 2.2
MFLX 1.9 ± 0.11 225 ± 7.96 12
STFX
3.7 ± 0.25
179 ± 10.7
4.8