Recovery (mean ± S.D., n = 3) of acetaminophen sulfate in apical and basolateral chambers of murine Bcrp1-MDCKII confluent cell monolayers Appearance of acetaminophen sulfate was quantified in both chambers after a 3-h incubation of cells with acetaminophen (10 μM) in either the apical or basolateral chamber as detailed under Materials and Methods.
Chamber Incubated with Acetaminophen | Presence of GF120918 (5 μM) | Acetaminophen Sulfate Recovery | Recovery Ratio Apical/Basolateral | ||
|---|---|---|---|---|---|
| Apical Chamber | Basolateral Chamber | ||||
| pmol | |||||
| Apical | - | 8.2 ± 0.3 | <1.5 | >5.5 | |
| Apical | + | 4.6 ± 0.7* | 3 ± 3 | 1.4 ± 0.7* | |
| Basolateral | - | 13 ± 1 | <1.5 | >8.4 | |
| Basolateral | + | 6.4 ± 0.4* | 6.8 ± 0.7* | 0.94 ± 0.06* | |
↵* P < 0.05, GF120918 vs. vehicle