List of oligonucleotide probes generated for use in bDNA signal amplification assay
Target Sequencea | Functionb | Probe Sequence |
|---|---|---|
| Cyp1A1 (NM_010902) | ||
| 1129-1152 | CE | gttcctcctggatctttctctgtaTTTTTctcttggaaagaaagt |
| 1217-1237 | CE | gtctccagaatgaaggcctccTTTTTctcttggaaagaaagt |
| 1386-1404 | CE | tttcaggccggaactcgttTTTTTctcttggaaagaaagt |
| 1486-1504 | CE | cggccaatggtctctccgaTTTTTctcttggaaagaaagt |
| 1505-1526 | CE | caggaagagaaagacctccgatTTTTTctcttggaaagaaagt |
| 1086-1106 | LE | catgaggctccacgagatagcTTTTTaggcataggacccgtgtct |
| 1107-1128 | LE | ccctagggttggttaccaggtaTTTTTaggcataggacccgtgtct |
| 1153-1177 | LE | cgatctctgccaatcactgtgtctaTTTTTaggcataggacccgtgtct |
| 1195-1216 | LE | agatagggcagctgaggtctgtTTTTTaggcataggacccgtgtct |
| 1346-1366 | LE | cggtcatggttaacctgccacTTTTTaggcataggacccgtgtct |
| 1367-1385 | LE | tgggtcaccccacagttccTTTTTaggcataggacccgtgtct |
| 1468-1485 | LE | tgcactttcgcttgcccaTTTTTaggcataggacccgtgtct |
| 1527-1545 | LE | tttgctgcagcaagatggcTTTTTaggcataggacccgtgtct |
| 1178-1194 | BL | cagaaagccggggctgc |
| 1238-1257 | BL | cgaaggatgaatgccggaag |
| 1258-1275 | BL | gggggatggtgaagggga |
| 1276-1300 | BL | agacttgtatctcttgtggtgctgt |
| 1301-1323 | BL | ccttggggatatagaagccattc |
| 1324-1345 | BL | tggttcacaaagacacagcacc |
| 1405-1422 | BL | cgctgggggtgagaaacc |
| 1423-1442 | BL | caagcgcttgtccagagtgc |
| 1443-1467 | BL | aaccaaagagagtgaccttctcact |
| Cyp2B10 (NM_009998) | ||
| 159-182 | CE | agctgaattaaagacttgaggaggTTTTTctcttggaaagaaagt |
| 205-224 | CE | ggtcccaggtgcactgtgaaTTTTTctcttggaaagaaagt |
| 286-303 | CE | ccggccagagaaagcctcTTTTTctcttggaaagaaagt |
| 969-993 | CE | aatctccttttggactttctctgtaTTTTTctcttggaaagaaagt |
| 1035-1053 | CE | gtatggcattttggtgcggTTTTTctcttggaaagaaagt |
| 1614-1633 | CE | cttggagccctggagatttgTTTTTctcttggaaagaaagt |
| 183-204 | LE | cacatcgccatatttttctcgaTTTTTaggcataggacccgtgtct |
| 225-245 | LE | cacagcataaccacaggccttTTTTTaggcataggacccgtgtct |
| 246-267 | LE | ctcccttatggtgtctgttccaTTTTTaggcataggacccgtgtct |
| 304-324 | LE | ctcaacgacagcaactgtcccTTTTTaggcataggacccgtgtct |
| 994-1014 | LE | gtgtgagccgatcacctgatcTTTTTaggcataggacccgtgtct |
| 1015-1034 | LE | tcatcaagggttggtagccgTTTTTaggcataggacccgtgtct |
| 1054-1075 | LE | tctcgtggatgactgcatctgaTTTTTaggcataggacccgtgtct |
| 1568-1587 | LE | ccaccagctgtctcagaggcTTTTTaggcataggacccgtgtct |
| 1588-1613 | LE | gagacatgcaataggagtactgatttTTTTTaggcataggacccgtgtct |
| 268-285 | BL | ggcttggcccaccagagc |
| Cyp3A11 (NM_007818) | ||
| 1094-1111 | CE | ggaggtgccttgttgggcTTTTTctcttggaaagaaagt |
| 1162-1185 | CE | caatgggatataatctgagggtttTTTTTctcttggaaagaaagt |
| 1266-1288 | CE | tcatggtgaagagcataagatggTTTTTctcttggaaagaaagt |
| 1379-1397 | CE | cctgggtccattcccaaagTTTTTctcttggaaagaaagt |
| 1586-1609 | CE | aagaactccttgagggagactcatTTTTTctcttggaaagaaagt |
| 1049-1071 | LE | gcagttttttctggatatcagggTTTTTaggcataggacccgtgtct |
| 1072-1093 | LE | agagcctcatcgatctcatcctTTTTTaggcataggacccgtgtct |
| 1112-1136 | LE | catctccatcacagtatcatacgtgTTTTTaggcataggacccgtgtct |
| 1137-1161 | LE | cattaagcaccatatccaggtattcTTTTTaggcataggacccgtgtct |
| 1216-1242 | LE | ggatatacacaccattgagttcaacatTTTTTaggcataggacccgtgtct |
| 1243-1265 | LE | aatcatcactgttgaccctttggTTTTTaggcataggacccgtgtct |
| 1353-1378 | LE | ggcagatatacataaggatcaatgctTTTTTaggcataggacccgtgtct |
| 1398-1417 | LE | aacctcatgccaaggcagttTTTTTaggcataggacccgtgtct |
| 1418-1441 | LE | gcaagtttcatattcatgagagcaTTTTTaggcataggacccgtgtct |
| 1541-1563 | LE | gtggcacaacctttagaacaatgTTTTTaggcataggacccgtgtct |
| 1564-1585 | LE | gctccagttatgactgcatcccTTTTTaggcataggacccgtgtct |
| Cyp4A14 (NM_007822) | ||
| 162-181 | CE | gggaaatgctggagggtcttTTTTTctcttggaaagaaagt |
| 220-240 | CE | tctgctggagctccttgtcctTTTTTctcttggaaagaaagt |
| 290-311 | CE | caggactcgtatattgctccccTTTTTctcttggaaagaaagt |
| 359-382 | CE | aattgataaattccagaagcctttTTTTTctcttggaaagaaagt |
| 143-161 | LE | gagcagccactgccttcgtTTTTTaggcataggacccgtgtct |
| 201-219 | LE | tcagatggtgcccccaaagTTTTTaggcataggacccgtgtct |
| 266-289 | LE | gagagacactgtaagcaggcacttTTTTTaggcataggacccgtgtct |
| 312-337 | LE | accttcacatagtcaggatcataaagTTTTTaggcataggacccgtgtct |
| 338-358 | LE | ggatctgatctccccagaaccTTTTTaggcataggacccgtgtct |
| 383-402 | LE | aaccaatccagggagcaaagTTTTTaggcataggacccgtgtct |
| 403-424 | LE | ccattcaacaggagcaaaccatTTTTTaggcataggacccgtgtct |
| 182-200 | BL | ccagtgggaaggcatgcat |
| 241-265 | BL | gggaatttctctacccatataagaa |
| Nqo1 (S75951) | ||
| 60-78 | CE | gccttggggtatgggctgaTTTTTctcttggaaagaaagt |
| 103-123 | CE | gagagatccttagggctggctTTTTTctcttggaaagaaagt |
| 208-225 | CE | ggctcttctcgccgccatTTTTTctcttggaaagaaagt |
| 272-292 | CE | cctctacagcagcctccttcaTTTTTctcttggaaagaaagt |
| 842-860 | CE | tcctcccagacggtttccaTTTTTctcttggaaagaaagt |
| 929-951 | CE | cttctgctcctcttgaacttcctTTTTTctcttggaaagaaagt |
| 952-974 | CE | acagagaggccaaacttgttcttTTTTTctcttggaaagaaagt |
| 36-59 | LE | gcgaaggctttatatcctgcttatTTTTTaggcataggacccgtgtct |
| 79-102 | LE | acaggctaggctagtaagagctgaTTTTTaggcataggacccgtgtct |
| 146-164 | LE | ggctgagcagctggttgccTTTTTaggcataggacccgtgtct |
| 165-186 | LE | atcgtaataccgaacgctgattTTTTTaggcataggacccgtgtct |
| 187-207 | LE | ggctccagatgttgagggaggTTTTTaggcataggacccgtgtct |
| 226-247 | LE | ctgaatgggccagtacaatcagTTTTTaggcataggacccgtgtct |
| 248-271 | LE | tggcgtagttgaatgatgtcttctTTTTTaggcataggacccgtgtct |
| 801-819 | LE | gatctgcatgcgggcatctTTTTTaggcataggacccgtgtct |
| 820-841 | LE | gacgtttcttccatccttccagTTTTTaggcataggacccgtgtct |
| 883-905 | LE | aagtttaggtcaaacaggctgctTTTTTaggcataggacccgtgtct |
| 906-928 | LE | ttttcattaagaatcctgcctgaTTTTTaggcataggacccgtgtct |
| 975-991 | LE | tgcccaggtgatggcccTTTTTaggcataggacccgtgtct |
| 124-145 | BL | aagacgaccctaaagctcttcg |
| 861-882 | BL | tggagcaaaatagagtggggtc |
CE, capture extender; LE, label extender; BL, blocker.
↵ a Target sequence refers to the region of the mRNA transcript to which a given probe is complementary. GenBank accession numbers for each transcript are given in parentheses next to the gene name.
↵ b Function refers to the type of bDNA oligonucleotide probe represented by each sequence.