Primers used in the present study Underlined letters indicated mutated nucleotides.
Primer | Sequence | Location |
|---|---|---|
| 2A6*9Sa | 5′–GATTCCTCTCCCCTGGAACC–3′ | 5′-UTR |
| 2A6int1ASa | 5′–TCCTGTCTTTCTGATGCTGA–3′ | Intron 1 |
| 2A6ex3 | 5′–TCCATCGCCACCCTGC–3′ | Exon 3 |
| 2A6*15 (MseI) | 5′–CTTTGGGGACCGCTTTGACTTT–3′ | Exon 4 |
| 2A6int4Ra | 5′–TGGAGACAGGGTATTGGACATC–3′ | Intron 4 |
| 2A6int4R2 | 5′–CTTGGAGACAGGGTATTGGA–3′ | Intron 4 |
| 2A6E8F-A | 5′–CCAGGGCACCGAAGTGTA–3′ | Intron 7-exon 8 |
| 2A6E8F-T | 5′–CCAGGGCACCGAAGTGTT–3′ | Intron 7-exon 8 |
| 2A6int8R | 5′–GAGGTTTAGATGTCCTTTCC–3′ | Intron 8 |
| 2A6R2b | 5′–AAAATGGGCATGAACGCCC–3′ | 3′-UTR |
| 2A6-ex8 mutant-S | 5′–GATTTCTTCCTCCCTAAGGGCACCGAAGTGTTCCCTATGCTG–3′ | Exon 7-exon 8 |
| 2A6-ex8 mutant-AS | 5′–CAGCATAGGGAACACTTCGGTGCCCTTAGGGAGGAAGAAATC–3′ | Exon 7-exon 8 |
| 2A6*7-SDM-S | 5′–CCCAGTCACCTAAGGACACTGACGTGTCCCCC–3′ | Exon 9 |
| 2A6*7-SDM-AS | 5′–GGGGGACACGTCAGTGTCCTTAGGTGACTGGG–3′ | Exon 9 |