TABLE 1

Kinetic parameters for each enzyme/substrate combination

Substrate	Enzyme Source	K_m	V_max	CL_int
		μM	nmol/min/nmol P450	ml/min/nmol P450
Diclofenac	Baculosomes	5.1 ± 0.9	29 ± 2	5.7
	Supersomes	2.6 ± 0.3	14.8 ± 0.4	5.7
	RECO	16 ± 2	16.8 ± 0.9	1.1
	Reconstituted	30 ± 3	27 ± 1	0.9
	HLM^a	4.0	19.6^b	4.9^b
(S)-Flurbiprofen	Baculosomes	16.2 ± 0.9	34 ± 1	2.1
	Supersomes	21.6 ± 0.8	11.7 ± 0.8	0.54
	RECO	120 ± 10	16.8 ± 0.5	0.14
	Reconstituted	45 ± 2	11.5 ± 0.2	0.25
	HLM^c	1.9	4.0^b	2.1^b
Tolbutamide	Baculosomes	103 ± 6	10.1 ± 0.2	0.098
	Supersomes	194 ± 29	4.0 ± 0.2	0.021
	RECO	1462 ± 50	5.6 ± 0.1	0.0038
	Reconstituted	220 ± 19	3.9 ± 0.1	0.018
	HLM^a	147	3.2^b	0.021^b
(S)-Warfarin	Baculosomes	4.6 ± 0.3	2.33 ± 0.03	0.51
	Supersomes	13 ± 2	0.38 ± 0.01	0.029
	RECO	32 ± 2	0.698 ± 0.009	0.022
	Reconstituted	52 ± 9	1.54 ± 0.07	0.030
	HLM^d	3.7	0.12^b	0.032^b

↵ a Walsky and Obach (2004).
↵ b Converted from literature values using an average CYP2C9 microsomal concentration of 85.4 pmol/mg (Wester et al., 2000).
↵ c Tracy et al. (1995).
↵ d Hemeryck et al. (1999).