Rata | Mouse | Doga | Monkey | |
---|---|---|---|---|
LTC4 | ||||
Transport activity (at 2.5 nM) (pmol/min/nmol Mrp2) | 2.57 ± 0.44 | 14.3 ± 0.8 | 9.76 ± 0.78 | 33.7 ± 0.1 |
Km (μM) | 2.05 ± 0.41 | 0.65 ± 0.26 | 0.53 ± 0.03 | 0.65 ± 0.19 |
Vmax (pmol/min/mg protein) | 1554 ± 51 | 1022 ± 86 | 1180 ± 8 | 2039 ± 168 |
V′max (pmol/min/pmol Mrp2) | 2.97 ± 0.10 | 3.92 ± 0.33 | 2.88 ± 0.02 | 6.56 ± 0.54 |
Hill coefficient | 1.16 ± 0.22 | 1.30 ± 0.04 | ||
V′max/Km (μl/min/pmol Mrp2) | 1.45 ± 0.29 | 6.03 ± 2.47 | 5.43 ± 0.31 | 10.1 ± 3.1 |
E217βG | ||||
Transport activity (at 25 nM) (pmol/min/nmol Mrp2) | 8.33 ± 0.86 | 3.57 ± 0.43 | 6.20 ± 0.96 | 3.58 ± 0.46 |
Km (μM) | 1.39 ± 6.50 | 2.13 ± 5.67 | ||
Vmax (pmol/min/mg protein) | 1192 ± 129 | 287 ± 41 | ||
V′max (pmol/min/pmol Mrp2) | 2.27 ± 0.34 | 0.70 ± 0.11 | ||
PS′ns (μl/min/pmol Mrp2) | 0.059 ± 0.007 | 0.064 ± 0.005 | 0.058 ± 0.002 | 0.084 ± 0.008 |
V′max/Km (μl/min/pmol Mrp2) | 1.63 ± 7.64 | 0.33 ± 0.88 | ||
BSP | ||||
Transport activity (at 70 nM) (pmol/min/nmol Mrp2) | 45.7 ± 7.1 | 9.51 ± 3.89 | 41.8 ± 7.7 | 41.8 ± 6.1 |
Km (μM) | 2.96 ± 0.91 | 5.35 ± 1.52 | 4.82 ± 0.47 | 4.77 ± 1.67 |
Vmax (nmol/min/mg protein) | 2.37 ± 0.19 | 1.30 ± 0.12 | 1.77 ± 0.05 | 1.83 ± 0.20 |
V′max (pmol/min/pmol Mrp2) | 6.04 ± 0.49 | 3.70 ± 0.33 | 4.90 ± 0.14 | 7.98 ± 0.86 |
V′max/Km (μl/min/pmol Mrp2) | 2.04 ± 0.65 | 0.69 ± 0.21 | 1.02 ± 0.10 | 1.67 ± 0.61 |
CCK-8 | ||||
Transport activity (at 3 nM) (pmol/min/nmol Mrp2) | 1.28 ± 0.16 | 0.77 ± 0.17 | 1.35 ± 0.41 | 1.30 ± 0.22 |
Km (μM) | 3.14 ± 0.73 | 2.37 ± 0.14 | 9.79 ± 0.89 | 5.73 ± 0.90 |
Vmax (pmol/min/mg protein) | 771 ± 41 | 469 ± 15 | 879 ± 25 | 526 ± 30 |
V′max (pmol/min/pmol Mrp2) | 1.97 ± 0.11 | 1.33 ± 0.04 | 2.44 ± 0.07 | 2.29 ± 0.13 |
Hill coefficient | 1.86 ± 0.17 | 1.18 ± 0.14 | ||
V′max/Km (μl/min/pmol Mrp2) | 0.63 ± 0.15 | 0.56 ± 0.04 | 0.25 ± 0.02 | 0.40 ± 0.07 |
↵ a Cited from our previous publication (Ninomiya et al., 2005).