TABLE 1

Kinetic parameters

Each value was calculated from the data shown in Fig. 4 (transport activity) and Fig. 4 (kinetic parameters). Values represent the obtained parameter ± computer-calculated S.D. V′max and PS′ns values mean, respectively, Vmax and PSns values normalized by the absolute expression level of each Mrp2 protein.

Rata
Mouse
Doga
Monkey
LTC4
    Transport activity (at 2.5 nM) (pmol/min/nmol Mrp2) 2.57 ± 0.44 14.3 ± 0.8 9.76 ± 0.78 33.7 ± 0.1
    Km (μM) 2.05 ± 0.41 0.65 ± 0.26 0.53 ± 0.03 0.65 ± 0.19
    Vmax (pmol/min/mg protein) 1554 ± 51 1022 ± 86 1180 ± 8 2039 ± 168
    V′max (pmol/min/pmol Mrp2) 2.97 ± 0.10 3.92 ± 0.33 2.88 ± 0.02 6.56 ± 0.54
    Hill coefficient 1.16 ± 0.22 1.30 ± 0.04
    V′max/Km (μl/min/pmol Mrp2) 1.45 ± 0.29 6.03 ± 2.47 5.43 ± 0.31 10.1 ± 3.1
E217βG
    Transport activity (at 25 nM) (pmol/min/nmol Mrp2) 8.33 ± 0.86 3.57 ± 0.43 6.20 ± 0.96 3.58 ± 0.46
    Km (μM) 1.39 ± 6.50 2.13 ± 5.67
    Vmax (pmol/min/mg protein) 1192 ± 129 287 ± 41
    V′max (pmol/min/pmol Mrp2) 2.27 ± 0.34 0.70 ± 0.11
    PS′ns (μl/min/pmol Mrp2) 0.059 ± 0.007 0.064 ± 0.005 0.058 ± 0.002 0.084 ± 0.008
    V′max/Km (μl/min/pmol Mrp2) 1.63 ± 7.64 0.33 ± 0.88
BSP
    Transport activity (at 70 nM) (pmol/min/nmol Mrp2) 45.7 ± 7.1 9.51 ± 3.89 41.8 ± 7.7 41.8 ± 6.1
    Km (μM) 2.96 ± 0.91 5.35 ± 1.52 4.82 ± 0.47 4.77 ± 1.67
    Vmax (nmol/min/mg protein) 2.37 ± 0.19 1.30 ± 0.12 1.77 ± 0.05 1.83 ± 0.20
    V′max (pmol/min/pmol Mrp2) 6.04 ± 0.49 3.70 ± 0.33 4.90 ± 0.14 7.98 ± 0.86
    V′max/Km (μl/min/pmol Mrp2) 2.04 ± 0.65 0.69 ± 0.21 1.02 ± 0.10 1.67 ± 0.61
CCK-8
    Transport activity (at 3 nM) (pmol/min/nmol Mrp2) 1.28 ± 0.16 0.77 ± 0.17 1.35 ± 0.41 1.30 ± 0.22
    Km (μM) 3.14 ± 0.73 2.37 ± 0.14 9.79 ± 0.89 5.73 ± 0.90
    Vmax (pmol/min/mg protein) 771 ± 41 469 ± 15 879 ± 25 526 ± 30
    V′max (pmol/min/pmol Mrp2) 1.97 ± 0.11 1.33 ± 0.04 2.44 ± 0.07 2.29 ± 0.13
    Hill coefficient 1.86 ± 0.17 1.18 ± 0.14
    V′max/Km (μl/min/pmol Mrp2)
0.63 ± 0.15
0.56 ± 0.04
0.25 ± 0.02
0.40 ± 0.07