Comparative expression of P450s and nuclear receptors mRNA in HepaRG cells, freshly isolated human hepatocytes, and HepG2 cells
Results are expressed as percentage compared to freshly isolated hepatocytes (FIH) arbitrarily set at 100%. FIH correspond to a pool of three different cell populations. Results are the mean of two independent experiments in duplicate.
CYP1A1 | CYP2B6 | CYP2C9 | CYP2D6 | CYP2E1 | CYP3A4 | PXR | CAR | PPARα | AhR | |
|---|---|---|---|---|---|---|---|---|---|---|
| FIH | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| HepG2 cells | 0.3 | 0.5 | 0 | 2.3 | 0 | 0 | 49.5 | 2.4 | 208.5 | 35.5 |
| HepaRG cells seeded at low density (days) | ||||||||||
| 5 | 0.1 | 0 | 0 | 1 | 0 | 0 | 6.8 | 0 | 56.5 | 94.5 |
| 8 | 0.1 | 0.1 | 0.1 | 1.2 | 0 | 0 | 8 | 0.1 | 126.8 | 134.3 |
| 15 | 0.3 | 3.2 | 3.3 | 0.9 | 0.1 | 4.5 | 51.3 | 4.1 | 175.6 | 121.7 |
| 30 | 2.3 | 3.5 | 9.5 | 1.2 | 0.2 | 8.1 | 95.7 | 9.1 | 187.2 | 64.2 |
| 30 (DMSO) | 9.1 | 34.6 | 34.7 | 0.8 | 3.5 | 176 | 142.9 | 22.2 | 346.5 | 169.7 |
| 35 (-DMSO 24 h) | 5.5 | 3.5 | 26.5 | 1 | 0.8 | 1.6 | 99.9 | 8.3 | 136.7 | 98.8 |
| 37 (-DMSO 72 h) | 3.9 | 1.6 | 16.7 | 0.9 | 0.8 | 2.3 | 122.6 | 7.1 | 135.1 | 47.5 |
| HepaRG cells seeded at high density after differentiation | ||||||||||
| + DMSO 24 h | 17.8 | 72.4 | 38.1 | 1.1 | 13.3 | 243 | 176.8 | 26.4 | 330.1 | 92.5 |
| - DMSO 24 h | 7.5 | 4.9 | 22.1 | 0.8 | 0.7 | 6.8 | 106.4 | 5.3 | 173.1 | 72.9 |
| + DMSO 72 h | 3.7 | 73.1 | 43.8 | 1 | 7 | 255.6 | 175.3 | 30.2 | 561.1 | 66.4 |
| - DMSO 72 h | 5.4 | 11.9 | 22 | 0.8 | 1 | 2.8 | 151.1 | 6.4 | 222.9 | 73.4 |