Comparative expression of phase 2 enzymes and liver-specific protein mRNA in HepaRG cells, freshly isolated human hepatocytes, and HepG2 cells
Results are expressed as percentage compared to freshly isolated human hepatocytes (FIH) arbitrarily set at 100%. FIH correspond to a pool of three different cell populations. Results are the mean of two independent experiments in duplicate.
GSTA1/2 | GSTA4 | GSTM1 | UGT1A1 | Aldolase B | Haptoglobin | Albumin | AFP | Thioredoxin | |
|---|---|---|---|---|---|---|---|---|---|
| FIH | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| HepG2 cells | 0.2 | 2.1 | 2.4 | 5.2 | 0 | 5.1 | 12.3 | 89,011 | 115.8 |
| HepaRG cells seeded at low density (days) | |||||||||
| 5 | 0.4 | 6.7 | 8.5 | 7.4 | 0 | 5.1 | 1.8 | 0.6 | 1250.6 |
| 8 | 8 | 5.9 | 7.6 | 9 | 0.2 | 76.4 | 14.1 | 21 | 877 |
| 15 | 37.3 | 9 | 9.8 | 10.5 | 7.8 | 199.9 | 85.6 | 75.6 | 422 |
| 30 | 36.8 | 13.1 | 15.5 | 17.1 | 20.9 | 256.6 | 82.9 | 79.4 | 303.4 |
| 30 (DMSO) | 108.6 | 22.6 | 26.3 | 186.3 | 13.5 | 216.4 | 70.7 | 45.8 | 304.4 |
| 35 (- DMSO 24 h) | 35.7 | 3.9 | 5 | 43 | 8.7 | 294.1 | 63.1 | 71.3 | 610.2 |
| 37 (- DMSO 72 h) | 18.3 | 5.8 | 6.8 | 35.4 | 9.6 | 344.7 | 77.9 | 64.8 | 479.7 |
| HepaRG cells seeded at high density after differentiation | |||||||||
| + DMSO 24 h | 258.2 | 26.4 | 33.6 | 240.6 | 16.3 | 197.5 | 57.1 | 40.2 | 429.8 |
| - DMSO 24 h | 50 | 4.2 | 5.8 | 82.4 | 7.3 | 344.8 | 47.2 | 54 | 790.9 |
| + DMSO 72 h | 160.3 | 36.7 | 45.1 | 221.1 | 14.9 | 196.6 | 60.4 | 36.9 | 280.9 |
| - DMSO 72 h | 31.3 | 9.7 | 7.5 | 47.3 | 9.4 | 479.8 | 65.2 | 114 | 460.9 |