TABLE 4

Expression of P450s and nuclear receptor mRNA in primary human hepatocytes

RT-qPCR analysis was performed on hepatocyte RNA samples prepared from primary hepatocytes from day 0 (freshly isolated hepatocytes) to day 5 of culture in the absence of DMSO. The experiment was performed on one cell population. Results are expressed as percentage compared to freshly isolated hepatocytes (arbitrarily set at 100%).


Days

CYP1A2

CYP2B6

CYP2C9

CYP2D6

CYP2E1

CYP3A4

PXR

CAR
0 100 100 100 100 100 100 100 100
1 15 29 18 20 14 22 95 7
3 2 8 48 9 2 6 160 14
5
12
4
58
60
11
30
121
29