TABLE 4

Identification of erlotinib metabolites in humans using the LC-MS/MS method


Metabolite

Retention Time

[M + H]+

Product Ion
min m/z
M2 12.1 414 370, 356, 338, 324, 312, 266
M3 (M16-C6H8O6) 12.6 586 410, 352, 320, 294, 266
M6 (CP-457493) 14.9 428 370, 338, 324, 312, 294, 284, 266
M7 15.3 430 372, 354, 340, 314, 296, 282, 268
M17 15.9 396 352, 338, 320, 306, 294, 266
M18 16.5 512 336, 304, 278
M8 (M13-C6H8O6) 16.8 556 380, 336, 322, 304, 278
M9 (M16-SO3) 17.1 490 410, 352, 320, 294, 266
M10 (M14-C6H8O6) 18.1 556 380, 336, 322, 304, 278
M11 18.8 394 336, 318, 304, 290, 276
M16 (CP-394356) 20.3 410 352, 320, 294, 266
M12 (CP-373943) 21.6 366 322, 304, 290, 278, 250
M13 (CP-373413) 24.0 380 336, 322, 304, 278, 250
M14 (CP-373420) 25.3 380 336, 322, 304, 278, 250
Erlotinib (CP-358774)
30.3
394
336, 304, 278, 250
  • CP-number, synthetic standard; C6H8O6, glucuronidation; SO3, sulfation.