Sulfation of raloxifene and 4-hydroxytamoxifen by human liver cytosols
Cytosols were prepared from several human livers and assayed for sulfation activity with 5 μM raloxifene or 4-OHT as substrate, as described under Materials and Methods. The values represent means ± standard deviation of three reactions.
Human Liver | Raloxifene Sulfation | 4-OHT Sulfation | |
|---|---|---|---|
| pmol/min/mg protein | |||
| 1 | 1.79 ± 0.04 | 1.87 ± 0.08 | |
| 2 | 1.21 ± 0.02 | 1.08 ± 0.02 | |
| 3 | 1.27 ± 0.07 | 2.02 ± 0.01 | |
| 4 | 1.87 ± 0.02 | 1.76 ± 0.08 | |
| 5 | 1.23 ± 0.05 | 1.63 ± 0.01 | |
| 6 | 1.71 ± 0.01 | 1.93 ± 0.06 | |
| 7 | 1.25 ± 0.01 | 0.84 ± 0.02 | |
| 8 | 0.47 ± 0.02 | 0.16 ± 0.01 | |