Kinetic parameters estimated from 13-DMT and 12-HT formation
Each reported parameter and computer-generated error estimate (numbers in parentheses, expressed as %CV) represents the average of values obtained from two separate experiments.
Vmax1 | Km1,u | CLint1.u | Vmax2 | Km2,u | CLint2,u | |
|---|---|---|---|---|---|---|
| nmol/min/nmol | μM | ml/min/nmol | nmol/min/nmol | μM | ml/min/nmol | |
| 13-DMT | ||||||
| rCYP3A4+b5 | 8.0 (5.6) | 0.21 (12.7) | 38 | |||
| rCYP3A5+b5 | 17.0 (2.4) | 0.21 (6.4) | 82 | |||
| rCYP3A4 | 0.1 (53.8) | 0.002 (86.8) | 58 | 7.8 (9.6) | 0.9 (24.8) | 10 |
| rCYP3A5 | 0.2 (88.0) | 0.003 (148.4) | 97 | 10.8 (25.9) | 0.6 (46.5) | 23 |
| 12-HT | ||||||
| rCYP3A4+b5 | 0.6 (12.7) | 0.29 (24.9) | 2 | |||
| rCYP3A5+b5 | 1.4 (9.2) | 0.35 (19.2) | 4 | |||
| rCYP3A4 | 0.02 (269.0) | 0.011 (295.5) | 1.6 | 0.3 (40.1) | 1.2 (85.5) | 0.4 |
| rCYP3A5 | 0.01 (79.4) | 0.005 (100.3) | 3.0 | 0.8 (41.7) | 1.1 (65.1) | 0.8 |