Substrate depletion rate constant obtained by fitting the first 100 s of each substrate depletion curve to a single-exponential decay and reaction velocities calculated from those rate constants, as well as the reaction velocities obtained by monitoring product formation
These values were used to generate Fig. 1, b and c. Recovered kdep and reaction velocities were obtained from fits to simulations.
[S] | kdep | Reaction Velocity | ||
|---|---|---|---|---|
| v = kdep [S] | v = d[P]/dt | |||
| 0.01 μM | 8.96 × 10-3 s-1 | 8.96 × 10-12 M s-1 | 8.54 × 10-12 M s-1 | |
| 0.1 μM | 8.89 × 10-3 s-1 | 8.89 × 10-11 M s-1 | 8.48 × 10-11 M s-1 | |
| 1 μM | 8.26 × 10-3 s-1 | 8.26 × 10-10 M s-1 | 7.90 × 10-10 M s-1 | |
| 10 μM | 4.78 × 10-3 s-1 | 4.78 × 10-9 M s-1 | 4.67 × 10-9 M s-1 | |
| 100 μM | 9.03 × 10-5 s-1 | 9.03 × 10-9 M s-1 | 9.00 × 10-9 M s-1 | |
| 1000 μM | 9.89 × 10-6 s-1 | 9.89 × 10-9 M s-1 | 9.89 × 10-9 M s-1 | |