Apical to Basal (A to B)a | Basal to Apical (B to A)b | Ratio (B to A/A to B)c | ||
---|---|---|---|---|
μl/mg protein | ||||
GPFX | ||||
GFP | 84.3 ± 14.6 | 91.4 ± 7.5 | 1.08 ± 0.21 | |
hBCRP | 25.4 ± 3.3 | 251 ± 38 | 9.87 ± 1.98**†† | |
mBcrp | 43.8 ± 6.5 | 178 ± 25 | 4.05 ± 0.82**† | |
GPFX + Ko143 | ||||
GFP | 102 ± 3 | 113 ± 2 | 1.10 ± 0.04 | |
hBCRP | 115 ± 8 | 135 ± 12 | 1.17 ± 0.13 | |
mBcrp | 108 ± 5 | 118 ± 5 | 1.09 ± 0.06 | |
UFX | ||||
GFP | 76.9 ± 15.6 | 47.8 ± 5.1 | 0.62 ± 0.14 | |
hBCRP | 90.7 ± 3.5 | 166 ± 25 | 1.84 ± 0.28** | |
mBcrp | 53.4 ± 7.4 | 78.3 ± 11.5 | 1.46 ± 0.30*† | |
UFX + Ko143 | ||||
GFP | 101 ± 15 | 70.0 ± 18.4 | 0.69 ± 0.21 | |
hBCRP | 114 ± 24 | 95.1 ± 30.2 | 0.84 ± 0.32 | |
mBcrp | 83.0 ± 11.2 | 38.6 ± 3.8 | 0.47 ± 0.08 |
↵ a Transcellular transport in the apical-to-basal direction at 180 min.
↵ b Transcellular transport in the basal-to-apical direction at 180 min.
↵* P < 0.05, significantly different from the corresponding parameters in GFP expressing cells.
↵** P < 0.01.
↵† P < 0.05, significantly different from the corresponding parameters with 1 μM Ko143.
↵†† P < 0.01.