TABLE 1

Transcellular transport of fluoroquinolones across MDCK II monolayers expressing transporters and inhibition of transcellular transport by Ko143

Data are taken from Figs. 1 and 2. Each value represents the mean ± S.E.




Apical to Basal (A to B)a

Basal to Apical (B to A)b

Ratio (B to A/A to B)c
μl/mg protein
GPFX
GFP 84.3 ± 14.6 91.4 ± 7.5 1.08 ± 0.21
hBCRP 25.4 ± 3.3 251 ± 38 9.87 ± 1.98**††
mBcrp 43.8 ± 6.5 178 ± 25 4.05 ± 0.82**
GPFX + Ko143
GFP 102 ± 3 113 ± 2 1.10 ± 0.04
hBCRP 115 ± 8 135 ± 12 1.17 ± 0.13
mBcrp 108 ± 5 118 ± 5 1.09 ± 0.06
UFX
GFP 76.9 ± 15.6 47.8 ± 5.1 0.62 ± 0.14
hBCRP 90.7 ± 3.5 166 ± 25 1.84 ± 0.28**
mBcrp 53.4 ± 7.4 78.3 ± 11.5 1.46 ± 0.30*
UFX + Ko143
GFP 101 ± 15 70.0 ± 18.4 0.69 ± 0.21
hBCRP 114 ± 24 95.1 ± 30.2 0.84 ± 0.32
mBcrp
83.0 ± 11.2
38.6 ± 3.8
0.47 ± 0.08
  • a Transcellular transport in the apical-to-basal direction at 180 min.

  • b Transcellular transport in the basal-to-apical direction at 180 min.

  • * P < 0.05, significantly different from the corresponding parameters in GFP expressing cells.

  • ** P < 0.01.

  • P < 0.05, significantly different from the corresponding parameters with 1 μM Ko143.

  • †† P < 0.01.