TABLE 3

P450 and GST catalytic activities in HSC from two donors


Donor

ERODa

Midazolamb

GST-CDNBa
pmol/min/mg pmol/h/106 cells nmol/min/mg
1 <2 0.094 93
2
<2
0.111
166
  • a CYP1A-mediated EROD and GST-CDNB activities were measured in S9 fractions as described under Materials and Methods. The practical detection limit for EROD assay was 2 pmol/min/mg S9 protein.

  • b CYP3A-dependent midazolam activities were assayed in cultured HSC as described under Materials and Methods.