Effect of temperature on CP-671,305 accumulation in sandwich-cultured cryopreserved human hepatocytes
Results are presented as the mean of two replicates from each of two separate experiments.
Compound | Uptakeapp (37°C) | Uptakeapp (4°C) | 4°C/37°C | |
|---|---|---|---|---|
| pmol/mg protein/10 min | Ratio | |||
| CP-671,305 (2 μM) | 205, 329 | 75, 35 | 0.37, 0.11 | |
| Taurocholate (2 μM) | 238, 320 | 45, 62 | 0.19, 0.19 | |
| Midazolam (2 μM) | 390, 606 | 467, 766 | 1.2, 1.3* | |
↵* The ratios greater than unity likely reflect the lower amount of midazolam at 37°C due to metabolism.