TABLE 1

Kinetic parameters of the transcellular transport of TGZS across the monolayers of MDCKII/OATP1B1 cells expressing GFP, mBcrp, and hBCRP

The data are represented by mean values ± S.E. of triplicate experiments. Statistical significance was analyzed by one-way analysis of variance followed by Tukey multiple comparison test.

Efflux Clearance
Flux Ratio (Basal-to-Apical/Apical-to-Basal)
Apical-to-Basal
Basal-to-Apical
μl/h/mg protein
GFP 15.1 ± 0.2 21.9 ± 0.8 1.45 ± 0.06
mBcrp 12.2 ± 3.9 43.9 ± 1.2*** 3.60 ± 1.15
hBCRP
7.57 ± 1.01**
70.5 ± 2.8***,†††
9.31 ± 1.30

  • ↵** P < 0.01 and *** P < 0.001 statistical differences in efflux clearances with those in GFP-expressed cells.

  • ↵††† P < 0.001 statistical difference in efflux clearances between mBcrp- and hBCRP- expressed cells.