Primer sequences used for the amplification of the BCRP gene fragment and the annealing temperatures used in the PCR
Name | Region | Primer Sequence (5′→3′) | Size | PCR Condition |
|---|---|---|---|---|
| base pair | Tm; °C | |||
| BCRP1P | Promoter | F: AACCCAGCTAGGTCAGACGA | 557 | 60.0 |
| R: TTTGAGTGGGCACAGCAC | ||||
| BCRP2P | Promoter | F: TTCCTAGGGTAGATGCAGCAG | 509 | 60.0 |
| R: CAGGGACAAGCCAAACACTC | ||||
| BCRP3P | Promoter | F: GTAGAGGCAGGGTTTCACCA | 559 | 60.0 |
| R: AAGTGATTGCGCATGTTCAG | ||||
| BCRP4P | Promoter | F: CGTGCCTGGCCTCTATGTAT | 572 | 60.0 |
| R: CTGACGCAGGCAGATCACT | ||||
| BCRP5P | Promoter | F: GCCACCACACCCAGTGTAAT | 518 | 64.7 |
| R: TGCAAAGTAAAAACAAATCAAAACC | ||||
| BCRP1E | Exon 1 | F: AGCTCGTCCCCTGGATGT | 516 | 54.0 |
| R: CCACCAACCTTTCCAGACAC | ||||
| BCRP2E | Exon 2 | F: CTGCTCATTGCCACACATTT | 400 | 54.0 |
| R: GCCAAAACCTGTGAGGTTCA | ||||
| BCRP3E | Exon 3 | F: GTCTCAAACTCCTGGCCTCA | 403 | 54.0 |
| R: GCGTTGCAAATGCTCAATAA | ||||
| BCRP4E | Exon 4 | F: TGGATTCAAAGTAGCCATGAGA | 402 | 54.0 |
| R: ATTCTCCCTGCCTTTTCACA | ||||
| BCRP5E | Exon 5 | F: GGTTCATCATTAGCTAGAACTTTACC | 403 | 54.0 |
| R: TGGAAAGCAACCATTTTTGA | ||||
| BCRP6E | Exon 6 | F: TCTTACAGGACTGGCACACG | 426 | 54.0 |
| R: CCTTCCCTACATTCTTACCTGCT | ||||
| BCRP7E | Exon 7 | F: TCAGGCTGAACTAGAGCAAACA | 387 | 60.0 |
| R: AGCACCAAATGGAACAAACA | ||||
| BCRP8E | Exon 8 | F: CATGGGAAGAAGAGAGAAAGAAA | 412 | 60.0 |
| R: CAAAAACACCAACAGCACTCA | ||||
| BCRP9E | Exon 9 | F: GGTGTTAGGGAAGCATCCAA | 413 | 54.0 |
| R: TGAAGCAGATGATAACAGAACCA | ||||
| BCRP10E | Exon 10 | F: GCCAAGCCATTGAGTGTTTA | 386 | 60.0 |
| R: TGGGCAACAGAGCATGAC | ||||
| BCRP11E | Exon 11 | F: CCACAACAATCCAAGACTGTG | 423 | 60.0 |
| R: GTAATCCTCCGGATCCCATC | ||||
| BCRP12E | Exon 12 | F: GGTCTAGCCCTGAGGATGTG | 403 | 64.7 |
| R: GAGTGCAAAATGGACAGGTG | ||||
| BCRP13E | Exon 13 | F: AGGGTGGTTGGAGAGTGGAT | 412 | 60.0 |
| R: AGCAGAGCCCCATTTACAGA | ||||
| BCRP14E | Exon 14 | F: TGAGTGTCTTGAGTAAGTGGAGAGA | 420 | 54.0 |
| R: GACTCCCCAGCCTTGTGTTA | ||||
| BCRP15E | Exon 15 | F: TCTTGATTGCCAGGGAAAAT | 404 | 60.0 |
| R: CGCGCACAACTCACTTTATG | ||||
| BCRP16E | Exon 16 | F: TGACGGATGCTAGGAATGAA | 430 | 64.7 |
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| R: CCCATGGTTACTGTCTGAGGA |
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