Clone | Km | Vmax | Vmax/Km | Ki |
---|---|---|---|---|
μM | nmol/min/unit | μl/min/unit | mM | |
1A9 | 59.8 ± 3.1 | 8.8 ± 0.4 | 147.3 ± 2.2 | 1.5 ± 0.1 |
19-1 | 173.1 ± 36.6* | 1.0 ± 0.1* | 5.9 ± 0.6* | 2.1 ± 0.3* |
19-2 | 68.9 ± 11.2 | 6.7 ± 0.5* | 98.7 ± 9.5* | 1.4 ± 0.2 |
19-3 | 59.8 ± 2.2 | 7.7 ± 0.3* | 128.7 ± 3.6* | 2.0 ± 0.2* |
49-1 | 36.4 ± 4.6* | 25.1 ± 0.6* | 695.1 ± 69.3* | 1.3 ± 0.2 |
49-2 | 46.3 ± 10.7* | 24.8 ± 0.4* | 560.2 ± 57.5* | 1.6 ± 0.1 |
49-3 | 51.3 ± 10.5 | 14.8 ± 0.6* | 297.2 ± 63.5* | 1.3 ± 0.2 |
69-1 | 81.2 ± 7.4* | 25.4 ± 2.2* | 313.4 ± 10.5* | 1.2 ± 0.1* |
69-2 | 70.3 ± 9.9* | 26.4 ± 3.1* | 377.0 ± 12.1* | 1.6 ± 0.3 |
69-3 | 90.4 ± 4.9* | 24.7 ± 0.7* | 273.8 ± 7.6* | 1.2 ± 0.1* |
↵* P < 0.01 compared with the single expression system.