TABLE 6

Kinetic parameters for propofol O-glucuronide formation by UGT1A9 in single and double expression systems

Data are means ± S.D. of three independent determinations.

Clone
Km
Vmax
Vmax/Km
Ki
μM nmol/min/unit μl/min/unit mM
1A9 59.8 ± 3.1 8.8 ± 0.4 147.3 ± 2.2 1.5 ± 0.1
19-1 173.1 ± 36.6* 1.0 ± 0.1* 5.9 ± 0.6* 2.1 ± 0.3*
19-2 68.9 ± 11.2 6.7 ± 0.5* 98.7 ± 9.5* 1.4 ± 0.2
19-3 59.8 ± 2.2 7.7 ± 0.3* 128.7 ± 3.6* 2.0 ± 0.2*
49-1 36.4 ± 4.6* 25.1 ± 0.6* 695.1 ± 69.3* 1.3 ± 0.2
49-2 46.3 ± 10.7* 24.8 ± 0.4* 560.2 ± 57.5* 1.6 ± 0.1
49-3 51.3 ± 10.5 14.8 ± 0.6* 297.2 ± 63.5* 1.3 ± 0.2
69-1 81.2 ± 7.4* 25.4 ± 2.2* 313.4 ± 10.5* 1.2 ± 0.1*
69-2 70.3 ± 9.9* 26.4 ± 3.1* 377.0 ± 12.1* 1.6 ± 0.3
69-3
90.4 ± 4.9*
24.7 ± 0.7*
273.8 ± 7.6*
1.2 ± 0.1*

  • ↵* P < 0.01 compared with the single expression system.