LC-MS/MS analytical parameters of diltiazem and metabolites
Analyte | Diltiazem | MA | MD | Alprazolam | |||
|---|---|---|---|---|---|---|---|
| Mobile phase gradient program: % B (min) | 10(0) → 10 (0.6) → 66 (3.2) → 66 (3.6) → 90 (4.2) → 90 (4.3) → 10 (4.4) | ||||||
| Analyte m/z transition | 415 → 178 | 401 → 178 | 387 → 178 | 309 → 205 | |||
| Mode | Positive | Positive | Positive | Positive | |||
| Capillary voltage (kV) | 3.1 | 3.1 | 3.1 | 3.1 | |||
| Cone voltage (V) | 40 | 69 | 69 | 50 | |||
| Desolvation temperature (°C) | 350 | 350 | 350 | 350 | |||
| Collision energy (eV) | 36 | 20 | 20 | 80 | |||
| Retention time (min) | 5.9 | 5.4 | 5.3 | 5.6 | |||