TABLE 6

Effect of perinatal low protein diets on hexobarbital sleep time, hexobarbital hydroxylase activity, and CYP2C12 protein expression in young adult rats

A hexobarbital sleep time test was conducted in 58-day-old male and female rats, whereas hexobarbital hydroxylase activity and CYP2C12 protein expression were measured in 65-day-old female rats. Values are means ± S.D., n = 7–8. Differences between low protein diet groups and the non-purified diet group were not evaluated.

Diets	Hexobarbital Sleep Time			Hexobarbital Hydroxylase Activity: Female^a	CYP2C12 Protein Expression: Female^b
Diets		Male	Female	Hexobarbital Hydroxylase Activity: Female^a	CYP2C12 Protein Expression: Female^b
	min			× 10³
M76	23 ± 5^†	75 ± 10	25.8 ± 9.6	1.22 ± 0.25
LM76	20 ± 2^†	60 ± 3*	22.9 ± 7.9	1.18 ± 0.17
C93	22 ± 4^†	68 ± 13	20.4 ± 6.3	1.25 ± 0.21
L93	21 ± 4^†	62 ± 5	20.4 ± 6.3	1.11 ± 0.03
NP	22 ± 2^†	69 ± 4	24.6 ± 7.5	1.13 ± 0.14

↵* Significantly different from its respective purified control on the same day.
↵† Significantly different from female offspring in the same diet group.
↵ a Expressed as the ratio of peak areas of metabolite to internal standard per minute per milligram of microsomal protein.
↵ b Expressed relative to the signal of the control sample that was run on every blot.