TABLE 2

Conditions for high-performance liquid chromatography-tandem mass spectrometry analysis


Compound

Mass Transition

Column

Initial Conditionsa

Final Conditionsa

Retention Time
min
Alovudine 245.1→126.9 Atlantis C18 5 × 2.1, 5 μb A 95%, B 5% A 10%, B 90% 1.04
R,S-Apomorphine 268.3→191.1 Atlantis C18 5 × 2.1, 5 μb A 95%, B 5% A 10%, B 90% 1.17
R,S-Cetirizine 389.2→200.9 Atlantis C18 5 × 2.1, 5 μb A 95%, B 5% A 10%, B 90% 1.70
Codeine 300.0→151.9 Atlantis C18 5 × 2.1, 5 μb C 100% C 80%, B 20% 2.05
CP-122721 381.4→160.1 Atlantis C18 5 × 2.1, 5 μb A 95%, B 5% A 10%, B 90% 1.51
Diazepam 285.2→154.1 Atlantis C18 5 × 2.1, 5 μb A 95%, B 5% A 10%, B 90% 1.86
Gabapentin 172.2→137.1 Atlantis C18 5 × 2.1, 5 μb A 95%, B 5% A 10%, B 90% 0.88
Morphine 286.0→152.0 Atlantis C18 5 × 2.1, 5 μb A 95%, B 5% A 10%, B 90% 1.45
M3G 462.0→285.9 Atlantis C18 5 × 2.1, 5 μb C 100% C 80%, B 20% 1.25
M6G 462.3→286.1 Atlantis C18 5 × 2.1, 5 μb C 100% C 80%, B 20% 1.41
Norfloxacin 320.3→276.2 Polaris C18 5 × 2, 5 μc A 95%, B 5% A 10%, B 90% 1.07
Oxycodone 316.0→240.9 Atlantis C18 5 × 2.1, 5 μb C 100% C 80%, B 20% 2.01
Thiopental
241.0→100.9
Hypurity C18 5 × 2.1, 5 μd
A 95%, B 5%
A 10%, B 90%
1.64
  • M3G, morphine-3-glucuronide; M6G, morphine-6-glucuronide.

  • a A, 2% acetonitrile in 0.2% formic acid; B, 0.2% formic acid in acetonitrile; C, 0.2% formic acid in deionized water.

  • b Waters Corporation, Manchester, UK

  • c Varian, Inc., Palo Alto, CA.

  • d Chromtech, Hägersten, Sweden.