Kinetic parameters Km (μM), Vmax [nmol/(min · mg)], Vmax/Km, kcat (min-1), and catalytic efficiency (kcat/Km, min · mg) for DOX reduction to DOX-OL by the specified purified aldo-keto reductases and carbonyl reductases
Km | Vmax | Vmax/Km | kcat | kcat/Km | |||||
|---|---|---|---|---|---|---|---|---|---|
| μM | nmol/(mg · min) | ml/(mg · min) | min-1 | 1/(min · mM) | |||||
| AKR1A1 | 247 | 1.1 | 0.004 | 0.04 | 0.168 | ||||
| AKR1B1 | Nonsaturated kinetics | ||||||||
| AKR1B10 | 311 | 2.8 | 0.009 | 0.10 | 0.324 | ||||
| AKR1C3 | 129 | 183.5 | 1.422 | 6.75 | 52.510 | ||||
| AKR1C4 | 281 | 1.2 | 0.004 | 0.04 | 0.158 | ||||
| CBR1 | 167 | 20.6 | 0.123 | 0.62 | 3.724 | ||||
| AKR1C1 | N.D. | <0.02 | N.D. | N.D. | N.D. | ||||
| AKR1C2 | N.D. | <0.02 | N.D. | N.D. | N.D. | ||||
| CBR3 | N.D. | <0.02 | N.D. | N.D. | N.D. | ||||
N.D., not determined.