TABLE 3

Correlation between Vmax and Jmax, for apical influx at various CLd, when CLint,met = 0.01 ml/min and Km = 0.5 μM CLinflux = CLefflux = CLint,sec = 0 and CLd1 = CLd2 = CLd3 = CLd4 = CLd; all the unbound fractions were set to unity.

Assigned Vmax
A → B
B → A
Jmax
Embedded Image *
Embedded Image *
β
Jmax
Embedded Image *
Embedded Image *
β
nmol/min μM μM nmol/min μM μM
Case 1 (CLd = 0.01 ml/min)
   0.05 nmol/min 0.0134 0.581 0.864 1.08 0.0285 14.8 14.6 1.01
   0.5 nmol/min 0.134 0.612 5.28 1.62 0.289 25.3 51.1 1.29
   5 nmol/min 1.34 1.38 34.1 1.82 331 1.24 × 105 1.24 × 105 1.00
   25 nmol/min –86.4 –1.02 × 104 N.A. N.A. 1.60 × 104 5.99 × 106 5.99 × 106 1.00
   50 nmol/min –157 –1.87 × 104 N.A. N.A. 6.86 × 104 2.56 × 107 2.56 × 107 1.00
Case 2 (CLd = 0.1 ml/min)
   0.05 nmol/min 0.00640 1.15 1.66 1.08 0.00825 1.40 1.28 0.99
   0.5 nmol/min 0.0640 1.34 5.22 1.39 0.0825 1.35 3.51 1.26
   5 nmol/min 0.642 2.66 28.7 1.73 0.826 2.01 19.4 1.70
   25 nmol/min –38.3 –6608 N.A. N.A. 6.91 343 374 1.08
   50 nmol/min –28.8 –5229 N.A. N.A. 1199 9.36 × 104 9.35 × 104 1.00
Case 3 (CLd = 1 ml/min)
   0.05 nmol/min 0.000671 1.60 1.61 1.00 0.000848 0.91 0.95 1.01
   0.5 nmol/min 0.00671 1.59 2.02 1.06 0.00848 0.93 1.15 1.04
   5 nmol/min 0.0671 1.70 5.95 1.36 0.0848 0.97 3.33 1.32
   25 nmol/min 0.336 2.39 18.3 1.65 0.424 1.18 11.1 1.69
   50 nmol/min
0.674
4.18
32.7
1.61
0.849
1.54
18.5
1.75

  • N.A., not available due to negative Jmax values

  • ↵* True Km is 0.5 μM