IBU enantiomer 2- and 3-hydroxylation, diclofenac 4′-hydroxylation (CYP2C9) and paclitaxel 6α-hydroxylation (CYP2C8) activity in HLM preparations from individual genotyped organ donors IBU enantiomer hydroxylation was measured in HLMs as described under Materials and Methods. Data represent means of duplicate determinations, which did not vary by more than 10%. The final concentration of each IBU enantiomer was 500 μM. For each HLM preparation, genotype, diclofenac hydroxylase and paclitaxel hydroxylase activities were provided by the supplier (BD Gentest).
HLM | Genotype | (R)-(–)-IBU | (S)-(+)-IBU | Diclofenac 4′-Hydroxylase | Paclitaxel 6α-Hydroxylase | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CYP2C9 | CYP2C8 | 2-OH | 3-OH | 2-OH | 3-OH | ||||||
| pmol/min/mg | |||||||||||
| HH40 | *1/*1 | *1/*1 | 168 | 170 | 121 | 163 | 243 | 76 | |||
| HH125 | *1/*1 | *1/*1 | 187 | 115 | 143 | 112 | 183 | 299 | |||
| HH55 | *1/*1 | *1/*1 | 254 | 201 | 181 | 185 | 288 | 396 | |||
| 4532891 | *1/*1 | *1/*1 | 206 | 189 | 146 | 186 | 281 | 250 | |||
| 4543196 | *1/*1 | *1/*1 | 215 | 212 | 146 | 203 | 287 | 200 | |||
| 4544547 | *1/*1 | *1/*3 | 202 | 185 | 130 | 163 | 266 | 250 | |||
| HH85 | *1/*2 | *1/*3 | 157 | 135 | 104 | 121 | 199 | 52 | |||
| 4522807 | *1/*2 | *1/*3 | 312 | 318 | 186 | 252 | 410 | 207 | |||
| HH131 | *1/*2 | *1/*3 | 224 | 147 | 142 | 129 | 206 | 237 | |||
| HH69 | *2/*2 | *3/*3 | 94 | 66 | 62 | 54 | 164 | 42 | |||
| 4532926 | *2/*2 | *3/*3 | 203 | 181 | 132 | 163 | 277 | 247 | |||
| 4538092 | *2/*3 | *1/*3 | 169 | 110 | 126 | 116 | 211 | 111 | |||