TABLE 4

Phenacetin O-deethylation rates and comparisons for CYP2A13 and mutations thereof All assays used 250 μM phenacetin.


Protein

Rate of Product Formationa

(Activitymutant/ActivityCYP2A13)
pmol/min/pmol
CYP2A13 3.39 ± 0.55 1.00
   L110V 0.40 ± 0.07 0.12
   A117V 17.84 ± 1.91 5.26
   S208I 0.35 ± 0.06 0.10
   A213S 1.48 ± 0.50 0.44
   F300I 0.07 ± 0.01 0.02
   A301G 0.18 ± 0.03 0.05
   M365V 0.49 ± 0.11 0.14
   L366I 11.41 ± 1.24 3.37
   G369S 0.36 ± 0.12 0.11
   H372R
1.06 ± 0.04
0.31
  • a Data represent the average of two independent experiments each conducted in triplicate