Kinetic parameters for CYP1A2, CYP2A13, and mutants of CYP2A13 and CYP2A6 Data represent the average of two independent experiments each with all concentrations conducted in triplicate.
Enzyme | Km | kcat | kcat/Km | [(kcat/Km)mutant/(kcat/K m)2A13] |
|---|---|---|---|---|
| μM | min–1 | μM–1 min–1 | ||
| 1A2a | 27.3 ± 2.2 | 9.68 ± 0.25 | 0.36 | 0.94 |
| 2A13 | 10.7 ± 2.0 | 3.82 ± 0.14 | 0.36 | 1.0 |
| A117V | 47.8 ± 5.8 | 29.4 ± 1.19 | 0.62 | 1.7 |
| A213S | 20.6 ± 2.5 | 2.14 ± 0.06 | 0.10 | 0.29 |
| M365V | 20.4 ± 4.7 | 0.93 ± 0.05 | 0.05 | 0.12 |
| L366I | 36.3 ± 4.5 | 13.7 ± 0.6 | 0.38 | 1.05 |
| H372R | 4.8 ± 1.3 | 1.91 ± 0.06 | 0.40 | 1.02 |
| 2A6 | N.D. | N.D. | N.D. | N.D. |
| I208S/I300F/G301A | 38.9 ± 7.0 | 0.98 ± 0.06 | 0.03 | 0.07 |
| I300F/G301A/S369G | 4.9 ± 0.5 | 0.89 ± 0.01 | 0.18 | 0.52 |
| I208S/I300F/G301A/G369S | 10.3 ± 0.8 | 2.88 ± 0.04 | 0.28 | 0.75 |