TABLE 1

Apparent permeability and permeability ratio of oseltamivir and Ro 64–0802 in LLCPK1-GA5-COL150 and LLC-PK1 cells (wild-type)

Condition
Cell Line
Papp, cm/s (× 10–6)
Permeability Ratio
Apical to Basal
Basal to Apical
mean ± S.E. mean ± S.E.
Oseltamivir LLCPK1-GA5-COL150 2.47 ± 2.47 19.2 ± 1.40 7.77
Wild-type 6.10 ± 0.18 7.24 ± 0.40 1.19
   With 10 μM CysA LLCPK1-GA5-COL150 6.06 ± 0.10 7.06 ± 0.60 1.17
Ro 64–0802 LLCPK1-GA5-COL150 1.55 ± 0.14 1.72 ± 0.14 1.11
   With 10 μM CysA
LLCPK1-GA5-COL150
1.73 ± 0.06
1.49 ± 0.07
0.86

  • Permeability ratio = (Papp, B to A)/(Papp, A to B); CysA, cyclosporin A

    The initial concentrations of test compounds were 100 μM

    Data are expressed as mean or mean ± S.E. of three experiments